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Comparison of the levels of proteins related to necroptosis‐related differentially expressed genes between the sepsis and control groups and between the deceased and surviving patients in the sepsis group (ELISA analysis).

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Comparison of the levels of proteins related to necroptosis‐related differentially expressed genes between the sepsis and control groups and between the deceased and surviving patients in the sepsis group (ELISA analysis).

Article Snippet: The Fas associated via death domain (FADD) levels were measured using the Human Protein FADD ELISA kit (CUSABIO, Wuhan, China).

Techniques: Comparison, Control, Enzyme-linked Immunosorbent Assay

Graphs showing receiver operating characteristic curves of proteins corresponding to the upregulated and downregulated necroptosis‐related genes for diagnosing sepsis in all study participants ( n = 145) (A) and predicting mortality in patients with sepsis ( n = 133) (B). Protein levels were measured using an enzyme‐linked immunosorbent assay. AUC, area under the curve; CYLD, Cylindromatosis Lysine 63 deubiquitinase; FADD, Fas associated via death domain; FasL, Fas ligand; NLRP6, NLR family pyrin domain containing 6; PYGL, glycogen phosphorylase L; TLR3, toll‐like receptor 3; TNF, tumour necrosis factor.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification and Verification of Necroptosis‐Related Genes in Patients With Sepsis by Bioinformatic Analysis and Molecular Experiments

doi: 10.1111/jcmm.70582

Figure Lengend Snippet: Graphs showing receiver operating characteristic curves of proteins corresponding to the upregulated and downregulated necroptosis‐related genes for diagnosing sepsis in all study participants ( n = 145) (A) and predicting mortality in patients with sepsis ( n = 133) (B). Protein levels were measured using an enzyme‐linked immunosorbent assay. AUC, area under the curve; CYLD, Cylindromatosis Lysine 63 deubiquitinase; FADD, Fas associated via death domain; FasL, Fas ligand; NLRP6, NLR family pyrin domain containing 6; PYGL, glycogen phosphorylase L; TLR3, toll‐like receptor 3; TNF, tumour necrosis factor.

Article Snippet: The Fas associated via death domain (FADD) levels were measured using the Human Protein FADD ELISA kit (CUSABIO, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay